CD8+, HLA-unrestricted, cytotoxic T-lymphocyte line against malignant melanoma

Table 3 Absence of Cytotoxicity blocking of CD8⁺ 793 CTL line against WM793 by MAbs

MAb^a			% WM793 target cell lysis	% Inhibition of WM793 target cell lysis^b
Designation	Specificity	Isotype	% WM793 target cell lysis	% Inhibition of WM793 target cell lysis^b
W6/32	HLA class I	IgG2a	27.9	-47.6
B33.1	HLA class II	IgG2a	23.8	-26.0
D1.B6	HLA class II	IgG1	21.0	-11.3
DX2	FAS (CD95)	IgG1	19.9	-5.3
OKT3	CD3	IgG2a	5.2	72.5^c
HI149	CD1	IgG1	23.6	-28.9
H24B5^d	Influenza virus	IgG2a	18.9	2.6
Normal mouse Ig^d	Unknown	Predominantly IgG1	18.3	3.2

^a Tumor targets were incubated with MAb (saturating concentrations, 10 μg/ml) for 1 hr at room temperature and excess MAb was removed before the 4-hr ⁵¹Cr-release assay.
^b Negative values indicate enhancement of lysis. Means of triplicate determinations at E:T ratios of 2.
^c Value significantly (p < 0.01) different when compared to isotype matched control antibody.
^d Isotype-matched control antibodies.

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