Figure 4

Protein complexes as identified by mass spectrometry in single spots. All proteins identified in one complex are drawn by a single color. A represents 4 of the 5 complexes found in trans-differentiated neuronal cells. Each complex contains brain-derived neurotrophic factor (BDNF*) and its interacting partner proteins. Each color in the diagram represents one complex of BDNF with other interacting proteins as identified by mass spectrometry. NOTE that all BDNF-interactive proteins in the 4 complexes in neuronal cells contain Kit tyrosine kinase. B displays proteins in 5 complexes derived from melanoma cells. NOTE that only one complex in melanoma cells contains Kit with no other protein in this complex.