Figure 3

Clinical outcome of patient #12 during exosome-based vaccination. This patient presented with progressive supraclavicular lymph nodes containing MAGE3 expressing tumor cells in July 2001 when enrolled in the exosomes Phase I trial starting in October 2001. She underwent a first leukapheresis for exosomes production and vaccination (weekly injections in Nov. 2001 during induction therapy and from January 2002 to April 2002 on a three week basis in continuation treatment). The initial size of the target LN are indicated on the left and were followed up by doppler pulsed ultrasonography from Nov 2001 to Dec 2001 (sizes indicated on the right side at the end of the induction therapy). Continuation therapy with exosomes was indicated and maintained clinical stability until the last available exosome dose in Jul. 2002, when she underwent surgery for lymphadenectomy. Results obtained by the pathologists are indicated (N+ if node is invaded by tumor cells, N- if not, MAGE3+ as expression of MAGE3 mRNA in RT-PCR). A second leukapheresis was performed on Aug. 2002 allowing a second therapy with exosomes on a 3 week basis that was continuated until Jul 2004. Six months after exosomes discontinuation (Oct 2004), the patient relapsed in contralateral LN and presented with one lung metastasis.