Figure 4
From: A modified human ELISPOT assay to detect specific responses to primary tumor cell targets
Medium containing HuAB serum enhanced the ability to measure tumor-reactive T cells. Pre- (white columns) and postvaccine (grey columns) PBMC (1 × 105 cells/well) were co-cultured with sCD40Lt activated autologous tumor cells (2 × 105 cells/well) for 48 h. The optimal media for the tumor IFN-γ ELISPOT assay was evaluated by comparing HuAB and FBS as the serum source. Although both HuAB and FBS containing media yielded significant (p < 0.001 and p < 0.05, respectively) differences between pre- and postvaccine T cell reactivity to tumors, medium containing HuAB yielded significantly (p < 0.05) more IFN-γ production.