Figure 3

CAM XTT assay. (A) Day 10 eggs (n = 10 eggs per group) were divided into four groups and received filter disks saturated with a vehicle solution (0.1% BSA/PBS) and systemic injection of carrier solution (0.1% DMSO) or bFGF filter disks and systemic injection of carrier solution, fumagillin or LM609. CAM disks in all groups then received topical XTT reagent at Day 12. Significant differences were observed between stimulated CAM disks and unstimulated disks (P = 0.028, Student's T test) after systemic injection of carrier solution and stimulated disks and CAM disks treated with systemic injection of fumagillin (P = 0.018, Student's t test) and LM609 (P = 0.029, Student's t test). (B) Day 10 eggs were divided into two groups (n = 6 eggs per group) and received filter disks saturated with bFGF followed 24 hours later by systemic injection of carrier vehicle alone (0.1% DMSO) or carrier containing CC5079. On Day 12 both groups received topical XTT reagent. A significant inhibition of endothelial cell proliferation was seen as a result of the systemic delivery of CC5079 compared to control (P = 0.0079, Mann-Whitney U Test).