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Table 2 Comparison between siRNA delivery methods.

From: RNA interference: learning gene knock-down from cell physiology

Method Advantages Drawbacks
Chemical or enzymatic synthesis Rapid
Enzymatic: no need to test individual siRNA
Chemical: high purity
Transient RNAi
Needs transfection
Enzymatic: variable purity & specificity
Chemical: expensive
DNA plasmid vector or casette Less expensive
Stable RNAi
Labor intensive
Needs transfection
Viral vector Stable RNAi
May be effective in cells resistant to transfection with dsRNA/plasmids
Labor intensive
Potential biohazard