From: RNA interference: learning gene knock-down from cell physiology
Method | Advantages | Drawbacks |
---|---|---|
Chemical or enzymatic synthesis | Rapid Enzymatic: no need to test individual siRNA Chemical: high purity | Transient RNAi Needs transfection Enzymatic: variable purity & specificity Chemical: expensive |
DNA plasmid vector or casette | Less expensive Stable RNAi | Labor intensive Needs transfection |
Viral vector | Stable RNAi May be effective in cells resistant to transfection with dsRNA/plasmids | Labor intensive Potential biohazard |