Table 2 Comparison between siRNA delivery methods.
From: RNA interference: learning gene knock-down from cell physiology
| Method | Advantages | Drawbacks |
|---|---|---|
| Chemical or enzymatic synthesis |
Rapid Enzymatic: no need to test individual siRNA Chemical: high purity |
Transient RNAi Needs transfection Enzymatic: variable purity & specificity Chemical: expensive |
| DNA plasmid vector or casette |
Less expensive Stable RNAi |
Labor intensive Needs transfection |
| Viral vector |
Stable RNAi May be effective in cells resistant to transfection with dsRNA/plasmids |
Labor intensive Potential biohazard |