Figure 3

Detection of PSMA protein by Western Blot. Total cell lysates were harvested and presence of PSMA protein was detected by anti-PSMA monoclonal antibody 4A12. A 100 kD band was identified in 3 clones with detectable PSMA mRNA but not in B16 cells transfected with empty vector (A). Lane1, 2 and 3, B16 cells transfected with pCDNA3.1-PSMA. Lane 4 B16 cells transfected with pCDNA3.1. β-actin was used as reference (B).