Forecasting the cytokine storm following systemic interleukin (IL)-2 administration

Table 4 Soluble factor (pg/ml) concentrations in the serum of 10 patients who completed the study (only factors that demonstrated significant changes in concentration after the 1^st dose compared with before therapy are shown)

Soluble Factor	Average before IL-2	STD error before IL-2	Average after 1 dose	STD error after 1 dose	p value before vs. after 1 dose	Bonferroni Correction
IL2*	0	0	3668	1178	0.00948	0.55953
TNFR1	1641	112	4702	512	0.00005	0.00292
MMP8	1187	455	3340	500	0.00008	0.00486
TNFR2	3195	259	6014	628	0.00010	0.00588
MCP1	984	227	81270	13486	0.00013	0.00787
IP10	146	18	1571	273	0.00047	0.02778
MCP2	32	9	1500	291	0.00050	0.02950
MMP9	233004	43617	736428	76301	0.00051	0.02984
I309	18	4	52	8	0.00057	0.03372
IFNγ	149	21	222	25	0.00059	0.03477
IL5	0	0	8	2	0.00094	0.05563
MIP1b	512	205	30149	6560	0.00097	0.05714
MIP3b	142	17	347	51	0.00108	0.06345
VegF	865	131	1392	181	0.00175	0.10307
IL7	2	1	10	2	0.00228	0.13444
IL15	0	0	6	2	0.00302	0.17834
E-Selectin	106029	5703	118981	5972	0.00351	0.20688
SDF1b	62	21	646	154	0.00372	0.21974
Lymph	111	22	259	39	0.00400	0.23580
IL6	50	5	747	201	0.00521	0.30754
MCP3	10	1	17	2	0.00616	0.36333
MMP1	12912	2478	17411	2235	0.00686	0.40490
Eotaxin	115	22	318	65	0.00762	0.44948
MIP1a	2952	143	3215	141	0.00790	0.46638
Angio2	494	78	655	101	0.01068	0.63039
MCP4	84	19	264	71	0.01082	0.63834
ITAC	29	4	110	26	0.01104	0.65159
TARC	248	59	478	112	0.02152	1.26942
IL8	61	4	456	151	0.02259	1.33290
IL4	0	0	5	2	0.02601	1.53464
TNFa	521	65	610	59	0.02824	1.66587
Gro-A	314	146	1401	468	0.03637	2.14567
TIMP2	75277	5629	85674	4973	0.04508	2.65947
KGF	373	117	470	97	0.04513	2.66289

Soluble Factors (pg/ml) present in the serum of patients undergoing high dose IL-2 immunotherapy were averaged before and after 1 dose of IL-2. A paired 2-tailed student t test was used to assess significance with an arbitrary cutoff of p= 0.05. Bonferroni's correction was applied to the data set by correcting for a total of 59 soluble factors (IL-2 was not factored in); IL-2 levels were included as internal control (*) since these values reflect in part administered human recombinant IL-2.

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