Forecasting the cytokine storm following systemic interleukin (IL)-2 administration

Table 3 Soluble factor (pg/ml) concentrations in the serum of 15 patients enrolled in the study (only factors that demonstrated significant changes in concentration after the 1^st dose compared with before therapy are shown)

Soluble Factor	Average before IL-2	STD error before	Average after 1 dose	STD error after 1 dose	p value before vs. after I dose	Bonferroni correction
IL2*	0	0	3269	841	0.00126	0.07409
TNFR2	3197	222	6215	462	0.00000	0.00001
MMP9	246408	29501	764715	53413	0.00000	0.00008
TNFR1	1659	92	5217	568	0.00000	0.00024
IFNγ	152	17	235	19	0.00000	0.00027
IP10	152	13	2006	267	0.00001	0.00030
MIP3b	151	12	421	45	0.00001	0.00040
MCP2	36	6	1776	261	0.00001	0.00045
MIP1b	525	137	35905	5761	0.00002	0.00100
MMP8	1502	321	3977	537	0.00002	0.00109
SDF1b	75	16	836	137	0.00005	0.00322
Lymph	142	20	338	43	0.00007	0.00407
IL7	3	1	10	2	0.00008	0.00477
IL5	1	1	9	2	0.00012	0.00691
MCP3	12	1	23	3	0.00020	0.01172
I309	22	4	73	12	0.00026	0.01547
E-Selectin	111526	5901	127683	5461	0.00030	0.01753
IL15	1	1	6	1	0.00038	0.02261
Eotaxin	130	16	401	65	0.00047	0.02757
ITAC	31	3	139	25	0.00056	0.03314
MIP1a	3000	111	3228	104	0.00102	0.06033
Gro-A	297	95	1848	388	0.00104	0.06121
Angio2	467	58	671	71	0.00107	0.06330
IL6	61	13	1092	265	0.00112	0.06589
VegF	1112	139	1498	132	0.00130	0.07661
MCP1	1327	248	124449	32058	0.00136	0.08025
MIG	262	35	933	178	0.00262	0.15480
IL8	65	4	800	211	0.00278	0.16379
IL4	0	0	4	1	0.00422	0.24896
TNFa	539	48	640	50	0.00719	0.42432
TARC	282	47	520	88	0.00939	0.55398
MIP3a	17	3	827	291	0.01195	0.70484
IL6R	24605	1759	27935	2652	0.01393	0.82204
IL10	2	1	41	15	0.01517	0.89505
MDC	605	77	661	74	0.03338	1.96915
MMP10	1440	148	1645	147	0.04876	2.87674
MMP1	13937	1775	16437	1602	0.05007	2.95398

Soluble Factors (pg/ml) present in the serum of patients undergoing high dose IL-2 immunotherapy were averaged before and after 1 dose of IL-2. A paired 2-tailed student t test was used to assess significance with an arbitrary cutoff of p= 0.05. Bonferroni's correction was applied to the data set by correcting for a total of 59 soluble factors (IL-2 was not factored in); IL-2 levels were included as internal control (*) since these values reflect in part administered human recombinant IL-2.

ISSN: 1479-5876