Volume 12 Supplement 1

Melanoma Bridge 2013: Poster and Oral Presentations

Open Access

In vivo targeting of cutaneous melanoma using an MSH-engineered human protein cage bearing fluorophore and MRI tracers

  • Luca Vannucci1,
  • Elisabetta Falvo2,
  • Cristina Maria Failla3Email author,
  • Miriam Carbo2,
  • Manuela Fornara4,
  • Rossella Canese5,
  • Serena Cecchetti5,
  • Lenka Rajsiglova1,
  • Dmitry Stakheev1,
  • Jiri Krizan1,
  • Alberto Boffi2, 4, 6,
  • Giulia Carpinelli5,
  • Veronica Morea2 and
  • Pierpaolo Ceci2
Journal of Translational Medicine201412(Suppl 1):P6

https://doi.org/10.1186/1479-5876-12-S1-P6

Published: 6 May 2014

Background

Nanoparticle (NP)-based materials are very promising agents for enhancing cancer diagnosis and treatment. Once functionalized for selective targeting of tumor expressed molecules, they can specifically deliver drugs and diagnostic molecules inside tumor cells.

Materials and methods

In the present work, we evaluated the in vivo melanoma-targeting ability of a nanovector (HFt-MSH-PEG) based on human protein ferritin (HFt), functionalized with both melanoma-targeting melanoma stimulating hormone (α-MSH) and stabilizing poly(ethylene glycol) (PEG) molecules. We used two independent and complementary techniques, such as whole-specimen confocal microscopy and magnetic resonance imaging, to detect the in vivo localization of NP constructs endowed with suitable tracers (i.e., fluorophores or magnetic metals).

Results

Targeted HFt-MSH-PEG NPs were shown to accumulate persistently at the level of primary melanoma and with high selectivity with respect to other organs. Melanoma localization of untargeted HFt-PEG NPs, lacking the α-MSH moiety, was less pronounced and disappeared after a few days. Further, HFt-MSH-PEG NPs accumulated to a significantly lower extent and with a different distribution in a diverse type of tumor (TS/A adenocarcinoma), which does not express α-MSH receptors. Finally, in a spontaneous lung metastasis model, HFt-MSH-PEG NPs localized at the metastasis level as well.

Conclusions

These results point at HFt-MSH-PEG NPs as suitable carriers for selective in vivo delivery of diagnostic or therapeutic agents to cutaneous melanoma.

Authors’ Affiliations

(1)
Institute of Microbiology, Academy of Sciences of the Czech Republic (ASCR), v.v.i.
(2)
Institute of Molecular Biology and Pathology, CNR – National Research Council of Italy
(3)
Molecular and Cell Biology Laboratory, IDI-IRCCS
(4)
Department of Biochemical Sciences “A. Rossi Fanelli”, University of Rome “Sapienza”
(5)
Department of Molecular and Cellular Imaging, Istituto Superiore di Sanità
(6)
Center for Life Nano Science at Sapienza, Istituto Italiano di Tecnologia

Copyright

© Vannucci et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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