Enrichment of KIR+CD57+ highly cytotoxic NK cells in sentinel lymph nodes of melanoma patients
- Talib Hassan Ali1, 2,
- Simona Pisanti3,
- Elena Ciaglia3,
- Roberta Mortarini4,
- Andrea Anichini4,
- Mario Santinami4,
- Elio Gulletta1,
- Caterina Ietto1,
- Mario Galgani5,
- Cinzia Garofalo1,
- Giuseppe Matarese5, 6,
- Maurizio Bifulco3,
- Soldano Ferrone7,
- Francesco Colucci8,
- Alessandro Moretta9,
- Klas Kärre10 and
- Ennio Carbone1, 10Email author
https://doi.org/10.1186/1479-5876-12-S1-P10
© Ali et al; licensee BioMed Central Ltd. 2014
Published: 6 May 2014
Keywords
Background
NK cells contribute to melanoma cell recognition and anti-tumor immunity, which is traditionally analyzed using human peripheral blood NK cells. An important checkpoint in the progression of malignant melanoma is the metastasis to lymph nodes.
Materials and methods
To investigate the role of lymph node NK cells in disease progression, we analyzed frequency, phenotype and functions of NK cells purified from either tumor infiltrated lymph nodes or tumor-free ipsilateral lymph nodes of the same patients. Lymph node NK cells were compared to peripheral blood NK cells from either melanoma patients or healthy donors.
Results
The data showed an expansion of CD56dimCD57+CD69+CCR7+KIR+ NK cells in tumor infiltrated lymph nodes. This phenotype corresponds to a recently described fully mature and highly cytotoxic NK cell population, and indeed we found that these lymph node NK cells displayed robust anti-tumor activity against autologous melanoma cells. The NK cells trafficking from periphery to the tumor draining lymph nodes have been investigated and the chemokines pattern identified. Moreover, the presence of a high proportion of KIR+CD57+CD56dim in the infiltrated lymph nodes was associated with an improved patients’ survival.
Conclusions
Our data suggest that NK cells from tumor infiltrated lymph nodes are attractive candidates to improve current NK cell-based immunotherapy of melanoma.
Authors’ Affiliations
Copyright
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
