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Revealing cancer initiating cells in metastatic melanomas by harnessing the host′s anti tumor humoral immune mechanisms

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Background

To determine whether the growth of tumors is sustained by the whole cancer cell population or it is maintained only by small fraction of cancer initiating cells, has crucial impact on the design of suitable therapies. That is why the research on defining the subpopulation of cancer initiating cancer stem cells (CSC) in solid tumors has come into highlights.

Materials and methods

A complex panel assay at cellular and molecular levels has been performed on primary and metastatic cancerous tissue biopsies and peripheral blood of patients with malignant melanomas (n = 153) (Ethycal permission: ETT TUKEB 16462- 02/2010).

Results

Cell cultures grew out of the great majority of the starter metastatic tissue specimen and cancer initiating cells could be sorted (BD FACSAvia Sorter) by colocalized unique sialilated glycosphingolipids and anti CD20 binding capacity. Characteristic growth pattern, spheroid forming, CSC markers like CD133, Nestin, ABCB5, CD20 and unique GD3 gangliosides were found. Patients′ sera and selected patients′ Epstein Barr Virus transformed cell supernatants in bulk or after limiting dilution cloning were tested for cancer binding by ELISA and immunofluorescence. Our novel tumor infiltrating B cell antibody phage display technique and DNA sequence cluster analysis (Vector NTI Advance 11, Bioedit 7.0, ClustalX2.0.11) resulted in some antibody fragments, belonging to representative tumor binding antibody variable region subgroups, with defined silalilated glycosphingolipid specificity.

Conclusion

Our strategy enables the detection and characterization of cancer stem cells in metastatic melanomas, with potential diagnostic importance. The novel peripheral blood and tumor infiltrating B cell antibody profile analysis proved to be a useful asset to reveal anti tumor humoral immune responsiveness and harness it by antibody engineering technique for further diagnostic and therapeutic usage.

Acknowledgements

The Harry J. Lloyd Charitable Trust Melanoma Research Award/ 2010, Fulbright No1206103 and OTKA T048933 Grants given to B. Kotlan are acknowledged.

Author information

Correspondence to Beatrix Kotlan.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Kotlan, B., Liszkay, G., Naszados, G. et al. Revealing cancer initiating cells in metastatic melanomas by harnessing the host′s anti tumor humoral immune mechanisms. J Transl Med 12, O1 (2014) doi:10.1186/1479-5876-12-S1-O1

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Keywords

  • Cancer Stem Cell
  • Cancer Stem Cell Marker
  • Anti Tumor
  • Antibody Phage Display
  • Virus Transformed Cell