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Table 3 Gene expression analysis of cancer pathways

From: In vitro biosafety profile evaluation of multipotent mesenchymal stem cells derived from the bone marrow of sarcoma patients

    MSC-SAR vs MSC-CTRL P03 MSC-SAR vs MSC-CTRL P10 U2OS vs MSC-CTRL P03
GENE SYMBOL Refseq Description Fold Regulation T-TEST p value Fold Regulation T-TEST p value Fold Regulation T-TEST p value
ANGPT2 NM_001147 Angiopoietin 2 3,60 0,017031 3,54 0,001587 2,01 0,123058
BRCA1 NM_007294 Breast cancer 1, early onset 1,56 0,184634 2,59 0,005080 13,19 0,000088
CDC25A NM_001789 Cell division cycle 25 homolog A (S. pombe) 2,01 0,030181 2,44 0,011524 15,56 0,000083
CFLAR NM_003879 CASP8 and FADD-like apoptosis regulator -3,43 0,013343 -1,91 0,017928 -1,42 0,115505
CHEK2 NM_007194 CHK2 checkpoint homolog (S. pombe) 1,03 0,930306 1,23 0,735103 7,49 0,000340
COL18A1 NM_030582 Collagen, type XVIII, alpha 1 -2,85 0,034861 -1,14 0,967097 2,94 0,018415
E2F1 NM_005225 E2F transcription factor 1 1,69 0,152813 2,64 0,036988 18,50 0,000019
GZMA NM_006144 Granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) 4,14 0,064832 3,86 0,021397 2,28 0,018937
IFNA1 NM_024013 Interferon, alpha 1 2,15 0,231676 4,32 0,000719 -4,17 0,208829
IGF1 NM_000618 Insulin-like growth factor 1 (somatomedin C) 3,98 0,023798 5,26 0,003415 1,41 0,704213
MMP9 NM_004994 Matrix metallopeptidase 9 (gelatinase B, 92 kDa gelatinase, 92 kDa type IV collagenase) 4,05 0,033049 2,76 0,957572 152,53 0,000000
PDGFB NM_002608 Platelet-derived growth factor beta polypeptide 2,41 0,057864 2,80 0,027891 174,40 0,000107
SERPINB5 NM_002639 Serpin peptidase inhibitor, clade B (ovalbumin), member 5 4,32 0,056954 3,54 0,010117 4,04 0,000712
TNF NM_000594 Tumor necrosis factor 4,04 0,030749 2,77 0,010705 1,54 0,285973
VEGFA NM_003376 Vascular endothelial growth factor A -1,97 0,006089 -6,84 0,008108 -12,44 0,000009
  1. Genes differentially expressed in MSC-SAR compared to MSC-CTRL at early and late passages characterized in the Human Cancer Pathway Finder PCR Array (RT2 Profiler PCR Array PAHS-033R, SABioscience), and analyzed using the provided RT2 Profiler PCR Array Data Analysis v. 3.5 software [32]. Results of the same genes analyzed in U2OS cells are reported for comparison. Bold characters indicate genes up- or down-regulated by ≥ 2-fold and with a p-value ≤ 0.05. The analysis was performed on four MSC-CTRL, four MSC-SAR samples and three independent samples of sub-confluent U2OS cells.