Figure 1

IFN γ ELISPOT assay testing CD8 ⁺ and CD4 ⁺ T cell responses to AFP and AdV. Patient PBMC from all time points were thawed and batch tested by direct IFNγ ELISPOT for recognition of autologous DC alone (background), DC transduced with AdVLacZ (for AdV responses) or co-cultured with AFP protein. Responses were also tested to HLA-A2 restricted peptides pulsed onto T2 cells (not shown). The spot counts after background (T cells + DC alone) per 10⁵ T cells is shown. A: Patient 7 responses are shown. Thin arrows indicate blood draws one month after each plasmid DNA injection and the thick arrow shows the blood draw one month after the AdV boostb. B: Patient 8 responses are shown as in 1A.