Observation of the different clones and detection of expression of IL-21, miR-200c and ZEB1 in B16F10 cells. (A-D, F, G, J, K) Morphological photos of B16F10 wild type (WT) clones, B16F10/GPI-IL-21 clones, B16F10/shZEB1 clones and B16F10/miR200c clones in order. The clones in left side were observed under a light microscrope, and the clones in right side were observed under a fluorescence microscrope. Magnification 400 × (A-D), and magnification 100 × (F, G, J, K). (D) The fluorescence on the surface of B16F10/GPI-IL-21 cells, but no fluorescence on the surface of B16F10 WT cells (B). (E) The positive band IL-21 in lane 2 in B16F10/GPI-IL-21 cells detected by western blot. (H, I) The expression of ZEB1 RNA and miR200c in shZEB1 stably transfected B16F10/shZEB1 cells and B16F10 WT cells detected by qRT-PCR. (L, M) The expression of miR200c and ZEB1 in lentivirus miR-200c stably transduced B16F10/miR200c cells and B16F10 WT cells detected by qRT-PCR.