LMFs can up-regulate CD163 expression on monocytes. (A, B) Monocytes were left untreated (MO) or were cocultured with normal skin fibroblasts (NF + MO) or LMFs (LMF + MO) for 6 days. Lightgray filled profile in B show the isotype control. The histograms are representative of six separate experiments. (C) Statistical analysis of the MFI of CD163 expression on monocytes following 6 days in coculture. The values shown in C represent the means ± SEM of six separate experiments. **(P < 0.01) and *(P < 0.05) indicate significant differences compared with the untreated monocytes or the monocytes cocultured with normal skin fibroblasts (C). Bar, 200 μm.