Epigenetic inactivation of mir-34b/c in addition to mir-34a and DAPK1 in chronic lymphocytic leukemia

Table 1 miR-34a , miR-34b/c and DAPK1 MSP Primer sequences and the reaction condition

Gene	Forward primer (5’ to 3’)	Reverse primer (5’ to 3’)	Tm/cycles/MgCl₂	References
*miR-34a*
U-MSP	GGGGATGAGGATTAGGATTTT	CAAACAAAACACATAAAAACAACA	58°C/35×/1.5 mM	[23]
M-MSP	GGGGATGAGGATTAGGATTTC	ACAAAACGCATAAAAACGACG	58°C/35×/1.5 mM
*miR-34b/c*
U-MSP	TTTTTATTTGTTTTGTTTTGTGTTTGTTTTG	CAACTACAACTCCCAAACAATCC	56.5°C/38×/2 mM	[27, 28]
M-MSP	ATTCGTTTCGTTTCGCGTTCGTTTC	CGACTACAACTCCCGAACGATCCG	60°C/34×/2 mM
*DAPK1*
U-MSP	GGAGGATAGTTGGATTGAGTTAATGTT	CAAATCCCTCCCAAACACCAA	63°C/35×/1.5 mM	[20]
M-MSP	GGATAGTCGGATCGAGTTAACGTC	CCCTCCCAAACGCCGA	63°C/35×/1.5 mM

Abbreviations: M-MSP MSP for the methylated allele, U-MSP MSP for the unmethylated allele, Tm annealing temperature.

ISSN: 1479-5876