Figure 5
Keratin staining of chronic-like TM perforations in plg -/- mice treated locally with plg. TM perforations in plg-/- mice were left untreated for 9 days to acquire the characteristics of chronic wounds. From day 10, plg was locally injected one or three times. The healing of the perforations was analyzed by IHC at day 19. (A) A representative section of a chronic TM perforation treated with three plg injections on days 10, 11, and 12 (Group 1); (B) A representative section of a chronic TM perforation treated with a single plg injection on day 10 (Group 2); (C) A representative section of a chronic TM perforation treated with three PBS injections (Group 3). The arrows indicate the perforated areas. Scale bar, 200 μm. EEC, external ear canal; MEC, middle ear cavity; TM, tympanic membrane.