Table 2 Luciferase assay vectors -overview
From: Chemokine receptor CCR6 expression is regulated by miR-518a-5p in colorectal cancer cells
Vector | Type | Example |
|---|---|---|
CCR6-3′UTR + luciferase gene (firefly) | Dual luciferase reporter assay system with unmutated regulatory region of CCR6 | Luc CCR6 vector (GeneCopoeia) CatalogNo: HmiT002212-MT01 |
No 3′UTR + luciferase gene (firefly) | Dual luciferase reporter assay without regulatory region of CCR6 | Luc No CCR6 vector (GeneCopoeia) CatalogNo: CmiT000001-MT01 |
Mutated CCR6-3′UTR + luciferase gene (firefly) | Dual luciferase reporter assay with mutated regulatory region of CCR6 | Luc CCR6 Mut vectors Lab construction vectors CatalogNo: HmiT002212-MT01 with point mutations in the miR-518a-5p seed sequences |
• Mutations in BS1 | • Luc CCR6 Mut BS1 | |
• Mutations in BS2 | • Luc CCR6 Mut BS2 | |
• Mutations in BS1 + 2 | • Luc CCR6 Mut BS1 + 2 |