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Table 2 Luciferase assay vectors -overview

From: Chemokine receptor CCR6 expression is regulated by miR-518a-5p in colorectal cancer cells

Vector Type Example
CCR6-3′UTR + luciferase gene (firefly) Dual luciferase reporter assay system with unmutated regulatory region of CCR6 Luc CCR6 vector (GeneCopoeia) CatalogNo: HmiT002212-MT01
No 3′UTR + luciferase gene (firefly) Dual luciferase reporter assay without regulatory region of CCR6 Luc No CCR6 vector (GeneCopoeia) CatalogNo: CmiT000001-MT01
Mutated CCR6-3′UTR + luciferase gene (firefly) Dual luciferase reporter assay with mutated regulatory region of CCR6 Luc CCR6 Mut vectors Lab construction vectors CatalogNo: HmiT002212-MT01 with point mutations in the miR-518a-5p seed sequences
• Mutations in BS1   • Luc CCR6 Mut BS1
• Mutations in BS2   • Luc CCR6 Mut BS2
• Mutations in BS1 + 2   • Luc CCR6 Mut BS1 + 2