Luciferase activity in miR transfected 293 T cells. Luciferase activity in 293 T cells cotransfected with Luc CCR6 and Luc No CCR6 vectors, respectively, and miR-518a-5p relative to negative control (NK) miR mimic transfected cells. Luciferase activities are presented as mean +/- SEM (n = 10). Co-transfection with hsa-miR-518a-5p resulted in significant down-regulation of luciferase activity *(P < 0.05) with respect to Luc No CCR6 and NK miR mimic transfected cells. Fold decrease below 1 indicates luciferase down-regulation in Luc CCR6/hsa-miR-518a-5p cotransfected tissues related to NK miR mimic transfected cells.