Figure 4

Effect of 5-HD on Δ ψ m and proliferation in PASMCs. (A) Morphology of isolated PASMCs. (A1) PASMCs appeared spindle- shaped, the typical “hill-and-valley” (phase contrast microscopy × 100); (A2) Positive expression of PASMCs α-actin appearance (Laser Confocal Microscope × 400). (B) Effect of mitochondrial ATP-sensitive potassium channel inhibitor (5-HD) on hypoxic depolarization of mitochondrial membrane potential in PASMCs treated with vehicle and normoxia as Controls, with vehicle and hypoxia (Hypoxia + V), with 5-HD and hypoxia (Hypoxia + 5-HD), or with the combination of 5-HD and Kv1.5 inhibitor (4-AP) and hypoxia (Hypoxia + 5-HD + 4-AP), measured by the intensity of R-123 fluorescence using a laser-scanning confocal microscope (B; ×200). (C) The proliferation of PASMC was measured by CCK-8 colorimetric assay. *and **stand for p values less than 0.05 and 0.01, as compared with Controls, respectively, and + stands for p values less than Hypoxia + V group.