Figure 4

Absence of CARPA reactions using the liposomal TRO40303 formulation. A) Serum from 10 healthy volunteers were incubated with either TRO40303 liposomal formulation at 5.5 mg/mL or AmBisome® at 4 mg/mL. After 45 min at 37°C, detection of SC5b-9 was performed by ELISA and the mean values were compared with basal level of the sera with no compound addition. The TRO40303 liposomal formulation did not induce SC5b -9 release whereas AmBisome® induce a significant release (***: p < 0.001 versus control). B) Systemic Arterial blood Pressure (SAP) and Pulmonary Arterial blood Pressure (PAP) changes were evaluated in a male pig after i.v. injection of the same volume of either saline or 10 mg/kg TRO40303 liposomal formulation infusion at 10 mg/mL followed by a bolus of Doxil® at 0.1 mg/kg. Doxil® induced an increase in PAP whereas TRO40303 had no effect. The data presented here are typical of 5 independent experiments.