Figure 2

Determination of VHHs’ Affinity, the establishment of DAS-ELISA and determination of assay properties. (A) The affinity of VHHs was determined by ELISA, VHHs were serially diluted into PBS (0.1 M, pH7.4) and PBS was used as the blank control. Each point represents the mean value of triplicate determinations. (B) Detection of HCC by paired test, the best concentration of coated 5 F2 was 5 ug/ml. Each point represents the mean value of triplicate determinations. (C) Detection of serum HCC by established DAS-ELISA, comparison of HCC level in serum samples between ELISA value and the standard value. The HCC concentration of the serum samples by the ELISA kit is 0.71 μg/ml, the accuracy is high. (D) Precision was measured by ELISA kit, the figure showed that intra-variability CV were 4.1%, 4.5%, 7.94% and 10.1%, in line with the requirements of CV <15%.