Figure 6

Inhibition of Aurora-A reverses migration ability of cisplatin-resistant cells. (A) A549 and A549/DDP cells were transfected with negative control (NC) or Aurora-A siRNA for 48 h and subjected to western blotting. Cells transfected with siRNA were treated with or without DDP (10 μM), and the cell migration ability was examined by wound healing assay. Images were captured at the time points as indicated. (B) A549 and A549/DDP cells were treated with DDP at 0 (control) and 10 μM, in combination with or without VX-680 (5 nM) or MLN8237 (0.2 μM). Then the cells were subjected to wound healing assay and relative wound closure quantified.