Upregulation of miR-208 promotes the proliferation ability of ESCC cells. A. Real-time PCR analysis of miR-208 expression in Kyse30 and Kyse410 cells stably expressing miR-208 and in control cells. B. Effects of ectopic miR-208 on the proliferation of Kyse30 and Kyse410 cells lines analyzed by the MTT assay. C. Representative micrographs (left) and quantification (right) of crystal violet stained cell colonies formed by the indicated ESCC cell lines, 10 days after inoculation. D. Effects of ectopic miR-208 on the tumorigenicity of Kyse30 and Kyse410 cell lines, as determined by an anchorage-independent growth ability assay. Colonies larger than 0.1 mm were scored. E. Effects of miR-208 overexpression on the cell cycle progression of ESCC cells measured by flow cytometry analysis. Each bar represents the mean ± SD of three independent experiments. *P <0.05.