Figure 3

Changes in KLF10 protein. Western blot analysis of KLF10 was performed with β-actin antibody as a loading control. Autoradiogram of the blot was scanned and quantified using an image processor program. A representative Western blot and the result of quantitative analysis are shown. A, A representative Western blot autoradiogram B, KLF10 protein increased after 24 weeks of high fat, sucrose diet (HFSD24) feeding and more so after 48 weeks of feeding (HFSD48). Mice that were given standard diet is designated as SD. C, KLF10 protein which was increased after 48 weeks of HFSD feeding, was attenuated when ursodeoxycholic acid (UDCA) was given with HFSD (HFSD/UDCA).