Figure 2

Histological assessment of inflammation and fibrosis in diet induced NASH. Histological analysis of the liver evaluating fatty change, inflammation, existence of hepatocyte ballooning and fibrosis was performed after H&E (X200) and Sirus red (X100) staining. A, Representative histological evaluation of mice fed with high fat, sucrose diet (HFSD). Liver specimens were obtained at 12, 24, or 48 weeks after the feeding. Mice fed with standard diet (SD) for 12 weeks served as the control. B, Representative histological assessment of mice fed with SD, HFSD and HFSD in combination with ursodeoxycholic acid (UDCA). Mice were fed with HFSD for 24 weeks and divided into two groups, from which one group was given HFSD with vehicle, and the other group was given HFSD with UDCA for another 24 weeks. Mice fed with SD and vehicle for 48 weeks served as the control.