Illustration of metrics used to quantify DDR responses. Two classes of metrics are calculated to measure distinct biological responses to modulation. The magnitude of the cellular responses is measured using (A) the Log2Fold metric while the fraction of cells responding is measured by the (B) Uu metric. (B) Examples of signaling responses for different Uu values are shown. Top to Bottom: Induced signaling will yield Uu values > 0.5; Lack of intracellular cellular response to modulation will yield Uu values ~ 0.5; Robust increases in signaling result in large magnitude shifts and a maximum Uu value of 1.0; Robust decreases in signaling result in large magnitude shifts with a minimum Uu value of 0.0.