Figure 3
MT3 is inactivated by promoter hypermethylation in AML cell lines. (A) MSP analysis of the methylation status of MT3 shows aberrant methylation in pediatric AML samples compared to NBM/ITP control samples. Aberrant methylation of MT3 was observed in 39.0% (16/41) of the pediatric AML samples compared to 10.0% (2/20) of the NBM control samples. M and U represent MSP results using primer sets for methylated and unmethylated MT3 genes, respectively. (B) BGS results of three AML samples and three NBM samples show that CpG islands are methylated in the AML samples and unmethylated in the NBM control samples. (C) Real-time PCR analysis of the transcript levels of MT3 in 41 pediatric AML samples and 20 NBM control samples. (D) Quantification shows that MT3 expression is significantly decreased in the AML samples compared to the NBM/ITP control samples. Furthermore, AML patients with methylated MT3 (n = 16) show lower MT3 transcript levels than those unmethylated MT3 (n = 25).