Figure 1

The periodicity of serum CRP concentration, and T _reg and T _eff frequency. Patients with gynaecological malignancies had blood collected 7 times over a period of 12 days. Serum was collected from 19 patients. While in 10 of the patients, PBMCs were also isolated. CRP levels in sera were determined along with the frequencies of Tregs and Teff within CD4⁺ T cells from PBMCs. The values for A, serum CRP concentration (n = 19, solid lines use the left Y axis while dashed lines use the right Y axis) (mg/L), B Treg and C, Teff frequencies (%, n = 10, solid lines use the left Y axis while dashed lines use the right Y axis) over time (days) were plotted in spaghetti plots. The periodicity for A, CRP concentration, B, Treg and C, Teff frequencies were assessed using the null hypothesis, that the population pointwise mean periodogram was a horizontal line at 1 (dotted line). The pointwise lower one-sided 95% confidence bound (dashed line) needed to exceed the null mark (dotted line) to suggest a significant peak.