Figure 8
From: Capillary nano-immunoassay for Akt 1/2/3 and 4EBP1 phosphorylation in acute myeloid leukemia
The Nanopro 1000 is capable of detecting signal using low cell numbers in primary AML samples. Three independent primary AML samples (A47316, A52453 and A52707) were used to test the capability of the assay to detect signal. Antibodies tested were Total 4EBP1 (A), Total Akt 1/2/3 (B), Phospho-4EBP1 Thr 37/46 (C) and Phospho-4EBP1 Ser 65 (D) Cell numbers ranged from 10,000-313 cells per capillary. Signal intensity (AUC, log-scale, y-axis) was plotted against cell numbers (log-scale, x-axis) and showed a linear increase with increasing cell numbers.