Telomere dysfunctional induced foci (TIF) in HBV-related multistep hepatocarcinogenesis and the correlations thereof with stathmin and elongation factor 1α (EF1α) expression. A. Representative features of colocalization of γ-H2AX and telomeric DNA in defined lesions of human multistep hepatocarcinogenesis. TIF are indicated by colored arrowheads: blue, DAPI; green, γ-H2AX; red, telomeres; yellow, TIF. B. Box plots show fractions of TIF-positive cells in normal liver (NL), liver cirrhosis (LC), low-grade dysplastic nodules (LGDN), high-grade dysplastic nodules (HGDN), and hepatocellular carcinoma (HCC). ‡ Statistical significance (linear trend model, P < 0.05). C. Correlation between TIF and TRF length. D. Correlation between TIF and histoscore EF1α. *Statistical significance (P < 0.05).