GOLPH3 regulates 5-FU sensitivity in CRC cells. (A and B) Western blot analysis of GOLPH3 expression in RKO and LoVo cells transfected with plasmid encoding full length of GOLPH3 or GOLPH3 siRNA pool (siG-pool). β-actin was used as a loading control. Cells were treated with different concentrations of 5-FU (0, 12.5, 25, 50, 100, 200 μM). And cell viability was analyzed by MTT assay. The growth inhibition ratio increased in GOLPH3-overexpressing CRC cells (C and E) and decreased in GOLPH3-silencing CRC cells (D and F) in a 5-FU dose-dependent manner. The values are mean ± SD of three independent experiments, *P < 0.05 compared with control cells.