Figure 5
From: Synergic silencing of costimulatory molecules prevents cardiac allograft rejection
Inhibitory function of Treg cells from tolerant recipients in MLR assays. Splenic T cells (2 × 105/well) from naïve BALB/C mice were used as responder cells. Cultured bone marrow DCs (1 × 105) from naïve C57BL/6 and C3H (third party) mice were used as stimulators. CD4+CD25+ cells from spleens of tolerant recipient mice were added to the cultures, the ratios of Treg cells compared with stimulators were 1:100, 1:20, 1:10. [3H]-thymidine incorporation was measured as described in Figure 1E. Inhibition rate was compare with a control where no inhibitor was added in the MLR. Data are presented as the mean ± SEM (*p < 0.01 C57BL/6 DCs vs C3H DCs, n = 6).