Figure 4

Treg cells in cardiac allograft recipients. (A) Flow cytometric analysis of Treg cells in BALB/C recipients with C57BL/6 grafts. T cells were isolated from spleens and lymph nodes of recipient at the time of allograft rejection or 100 days post transplantation. T cells were stained with antibodies against FoxP3, CD25 and CD4. Flow cytometry was performed to determine the percentages of Treg cells by first gating on CD4⁺ cells and then subsequently analyzing the percentages of CD25⁺ and FoxP3⁺ cells in the spleen and lymph nodes of recipients (A). (B & C) FoxP3 expression in splenocytes of recipients. T cells were isolated from spleens of recipient mice at the time of allograft rejection or 100 days post transplantation. Total RNA was extracted and transcripts of FoxP3 were determined using RT-PCR (B) and quantitative RT-PCR (C). (*p < 0.01 vs control groups).