Figure 3

Figure 3 The -26-exon FAK protein is resistant to cleavage by caspase-3/-7. A. The -26-exon FAK was found to be resistant to proteolysis in MCF-7 cells induced with TNF-α. MCF-7 cells transfected with wild-type or -26-exon HA-FAK were treated with TNF-α for different periods, and then the FAK protein was immunoprecipitated and analyzed. B. Statistical analysis of the relative normalized expression levels of FAK in the wild-type group compared with the -26-exon group, as shown in A. Compared with the normalized -26-exon group, the expression levels of FAK in the wild-type group were notably decreased (*P < 0.05). C. The -26-exon FAK was found to be resistant to proteolysis in MCF-10A cells cultured in serum-free medium. MCF-10A cells transfected with wild-type or -26-exon FAK were cultured in serum-free medium for 12 h, and then the expression levels of relative protein were examined. D. Statistical analysis of the relative expression levels of activated FAK and Akt in the wild-type group cultured in serum-free medium for 12 h compared with -26-exon group, as shown in C. Compared with the normalized -26-exon group, the expression levels of activated FAK and Akt in the wild-type group were notably decreased (*P < 0.05). E. The -26-exon FAK protein inhibits the activation of caspase-3/-7. MCF-10A cells transfected with wild-type or -26-exon FAK were cultured in serum-free medium for 12 h and then harvested and analyzed using anti-caspase-3 or anti-caspase-7 antibody. F. Statistical analysis of the relative expression levels of cleaved caspase-3 and caspase-7 in the -26-exon group cultured in serum-free medium for 0 h or 12 h compared with the wild-type group, as shown in E. Compared with the wild-type group, the levels of cleaved caspase-3 and -7 in the -26-exon group were significantly decreased (*P < 0.05).