Figure 2

UC-MSC phenotype analysis. MSCs derived from umbilical cord were detached with trypsin-EDTA and stained with fluorescence antibodies against surface molecules indicated and analyzed by flow cytometry present in histogram plots. Appropriate isotype controls were performed (for each antibody isotype) to assess cell auto-fluorescence and background staining. MSCs were positive for CD73 (99%), CD90 (99%), CD105 (95%), CD166 (98%), negative for hematopoietic marker CD34 (1.7%), leukocyte common antigen CD45 (2.2%), HLA-DR (1.3%) and monocyte marker CD14 (1.5%).