Figure 4

Detection of sex hormones and parasite load in uninfected and T. gondii -infected villous explants, treated or not with azithromycin or PSA. Estradiol (A), progesterone (B) and HCG + β (C) were measured by an electrochemiluminescence immunoassay of uninfected or T. gondii- infected villous explants. Levels of (D) estradiol, (E) progesterone, and (F) HCG + β in azithromycin- or PSA-treated or untreated villous explants were analyzed in relation to parasite load. Data were analyzed by ANOVA and Bonferroni multiple comparison post hoc test. Comparisons are represented by symbols: parasitism, ^***P < 0.001, ^**P < 0.01, ^*P < 0.05 and sexual hormones, ^###P < 0.001, ^##P < 0.01, ^#P < 0.05 in relation to NT control.