Intratumoral delivery of recombinant vaccinia virus encoding for ErbB2/Neu inhibits the growth of salivary gland carcinoma cells

Table 2 Immunoreactivity of rV- neu T vaccinated BALB- neu T mouse sera with Neu

Dose of rV- *neu* T	Number of mice with immune response/total^a	Number of pooled sera	Serum titer (SD)^b
10⁸ pfu	8/8	5	11875^c (1250) p = 0.000288^d p = 0.0045^e
10⁷ pfu	4/4	4	1950 (1706)
10⁶ pfu	6/6	5	3380 (3750)

^aImmune response was determined by ELISA against LTR-Neu and NIH3T3. Optical density (O.D) of V-wt sera at 1:250 to LTR-Neu was ≤0.3. These values were considered negative. Values for rV-neu T serum was considered positive when specific O.D value was ≥0.5 at the 1:1000 dilution.
^bImmune sera titers of BALB-neu T vaccinated mice were determined by ELISA against LTR-Neu and NIH3T3 using individual serum at different dilutions (1:1000, 1:2500, 1:12500). Sera titer represents the mean value of individual serum titer ± standard deviation (SD).
^cTiter was estimated as the highest immune serum dilution generating a specific absorbance of 0.5 at 492 nm.
^d10⁸ pfu vs 10⁷ pfu.
^e10⁸ pfu vs 10⁶ pfu.

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