aImmune response was determined by ELISA against LTR-Neu and NIH3T3. Optical density (O.D) of V-wt sera at 1:250 to LTR-Neu was ≤0.3. These values were considered negative. Values for rV-neu T serum was considered positive when specific O.D value was ≥0.5 at the 1:1000 dilution.
bImmune sera titers of BALB-neu T vaccinated mice were determined by ELISA against LTR-Neu and NIH3T3 using individual serum at different dilutions (1:1000, 1:2500, 1:12500). Sera titer represents the mean value of individual serum titer ± standard deviation (SD).
cTiter was estimated as the highest immune serum dilution generating a specific absorbance of 0.5 at 492 nm.