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Table 2 Immunoreactivity of rV- neu T vaccinated BALB- neu T mouse sera with Neu

From: Intratumoral delivery of recombinant vaccinia virus encoding for ErbB2/Neu inhibits the growth of salivary gland carcinoma cells

Dose of rV- neu T Number of mice with immune response/totala Number of pooled sera Serum titer (SD)b
108 pfu 8/8 5 11875c (1250) p = 0.000288d p = 0.0045e
107 pfu 4/4 4 1950 (1706)
106 pfu 6/6 5 3380 (3750)
  1. aImmune response was determined by ELISA against LTR-Neu and NIH3T3. Optical density (O.D) of V-wt sera at 1:250 to LTR-Neu was ≤0.3. These values were considered negative. Values for rV-neu T serum was considered positive when specific O.D value was ≥0.5 at the 1:1000 dilution.
  2. bImmune sera titers of BALB-neu T vaccinated mice were determined by ELISA against LTR-Neu and NIH3T3 using individual serum at different dilutions (1:1000, 1:2500, 1:12500). Sera titer represents the mean value of individual serum titer ± standard deviation (SD).
  3. cTiter was estimated as the highest immune serum dilution generating a specific absorbance of 0.5 at 492 nm.
  4. d108 pfu vs 107 pfu.
  5. e108 pfu vs 106 pfu.