Figure 4

Cellular anti-tumor response. (A) Cytotoxic T lymphocyte (CTL) response in patient 7. Peripheral blood mononuclear cell cytotoxicity was determined by flow cytometry analysis against bone marrow cells comprising MM cells using an apoptosis detection kit. Target cells were taken from bone marrow biopsy samples from patient 7 before the first vaccination. PBMCs were collected 4 weeks after the first vaccination with 1.25 mg phage Id vaccine. E:T: effector/target cell ratio. (B) Skin biopsy oft the site of phage Id challenge of patient 7. CD8+ (red), but no CD4+ T cell infiltrate was demonstrated.