Figure 3

miR- 182 facilitated tumor growth, invasion and metastasis in vivo . (A) SW480 cells were infected with pLV-miR-182 or empty vector virus and injected subcutaneously into nude mice. At 30 days after subcutaneous injection, SW480/miR-182 and SW480/miR-con cells produced primary tumors. (B) Representative figure of tumor formed (upper) and growth curve of tumor volumes (lower). Each data point represented the mean ± SD. (C) Representative photographs of H&E and immunohistochemical staining for Ki-67 antibody of primary cancer tissues. (D) The whole-body images of metastasis in nude mice. P denotes primary tumor. White arrows point to the metastatic nodules. (E) Histological images of invasion and metastatic nodules in organs. (a) metastasis of lymph node; (b) metastasis of liver; (c) metastasis of lung; (d) seeding metastasis of abdominal wall. (F) Incidence of metastasis in mice that were implanted with SW480/miR-182 or miR-con cells.