Figure 2

XB130 knockdown inhibited cell cycle progression and DNA synthesis by GC cells. (A) XB130 silencing arrested cell cycle progression in both cell lines, as indicated by accumulation of cells in G1 phase and reduced progression into S phase. Cell cycle analysis was performed by flow cytometry using propidium iodide staining. (^# p < 0.01 versus Scramble cells, n = 3). (B) The BrdU uptake assay showed a decrease of DNA synthesis in sh-XB130 transfected cells (newly synthesized DNA is stained red by BrdU, while nuclei are stained blue by 4′, 6-diamidino-2-phenylindole). *p < 0.05 and ^# p < 0.01 versus Scramble cells (n = 3 per group).