Figure 2

Ad-ANXA1 transfected into PMVECs was strongly expressed and the protein was found in both the cytoplasm and nuclei. (A) At 24 h after transfection with Ad-ANXA1, PMVECs had a normal structure with no observable pathological characteristics (magnification 100×). (B) Bright green fluorescence was observed in nearly all cells using an inverted fluorescence microscope following transfection with Ad-ANXA1 containing the green fluorescent protein (GFP) gene (magnification 100×). (C.D) The expression of the ANX A1 mRNA and protein in PMVECs 24 h after either the mock transfection or transfection with Ad-ANXA1 was determined by RT-PCR and western blotting. GAPDH was used as an internal control. (E) The mRNA and protein density of the ANX A1 gene for each group is displayed. Each data point represents the mean ± S.E.M. of four independent experiments. * p < 0.05; N: Non-transfected group; T: transfected group; E: empty vector group.