Figure 3

Effects on cellular invasiveness/migration and MMP-9/MMP-2 expressions of HO-8910 cells after transfection with basigin-2. HO-8910 cells were either untransfected (blank), or transfected with pcDNA3.1 (control) or pcDNA3.1-basigin-2 (basigin-2). A-B Western blot demonstrated basigin-2 protein levels were significantly increased after transfection with basigin-2. C-D HO-8910 cells were incubated for 24 h and conditioned media were used for the measurement of MMP-2 and MMP-9 protein levels by gelatin zymography. Zymographic analysis showed increased MMP-2 and MMP-9 activity in basigin-2-transfected HO-8910 cells. E-F Wound healing assay was performed on monolayers of HO-8910 cells transfected with pcDNA3.1-basigin-2. The wound gaps were measured at six reference points along the wound, and the results were expressed as the average wound gap. G-H Overexpression of basigin-2 in HO-8910 cells resulted in an increase in cellular migrate capacity. I-J Overexpression of basigin-2 in HO-8910 cells resulted in an increase in cellular invasive capacity. Scale bars = 0.5 mm (E). Scale bars = 0.1 mm (G, I). Statistical significance was assessed using one-way ANOVA. Data are presented as mean ± SD for three independent experiments. *P < 0.05, statistically significant difference.