Figure 4

Fluorescence microscopy of virus-mediated Ruc -GFP expression in HCT-116. HCT-116 cells were infected at an MOI of 1.0 and monitored for 72 hours. (BF) shows bright field microscopic images of the morphology of virus-infected cells. (GFP) expression in infected cells was visualized by direct fluorescence microscopy; propidium iodide (PI) was used as a marker for dead cells. Colocalization of (GFP) and (PI) signal is shown in the (merged) images. All pictures were taken at 40x magnification.