Figure 3

CRH expression and serum IL-6 levels in non-tumor- and B16-F10 (metastases)-bearing mice. Measurements were performed in non-tumor-bearing and in tumor-bearing mice (7 days after inoculation). mRNA expression of CRH in the hypothalamic PVN was evaluated as described under Materials and methods. CRH expression data (optical density arbitrary units, AU), measured at 0 h (circadian time, see Figure 1), are expressed as mean values + S.D. (error bars) of 6–7 different animals. For IL-6 levels determination blood was collected from the tail vein every 6 h during the 24-h period of the indicated day, and data are mean values of the peak serum cytokine concentrations + S.D. (error bars) (pg/ml) measured in 7–8 different animals. *P< 0.01 comparing B16-F10-bearing mice versus non-tumor-bearing mice.