Figure 6

CFL2 and breast cancer cell migration. (A) Bar graph representing the normalized number of migratory or invaded cells after the respective siRNA transfection. (B) Overexpression of CFL2 can compensate the effect of exogenous miR-200c. Bar graph represents the normalized number of migratory cells after the respective transfection of miRNA mimic control, miR-200c mimic, and co-transfection of miR-200c and a plasmid encoding CFL2, respectively. *: Student’s T-test, p < 0.05. (C). Rhodamin-phalloidin staining of F-actin in the CFL2-siRNA and miR-200c transfected MDA-MB-231 cells. (D) Immunohistochemical analysis of TMA of primary breast tumors using antibodies against CFL2. Representative images of TMA cores are presented showing normal breast tissue, grades I, II, and III breast tumors with increasing staining intensities. (E) Bar graph representing the quantification of CFL2 immunostaining from blindly scored tissue microarray sections. Staining intensity of each sample was given a modified histochemical score (MH-score) that considers both the intensity and the percentage of cells stained at each intensity level. The intensity of each grade is the average of MH-score of all samples in that grade. Data were analyzed by one-way ANOVA with p-values as noted in the figure.