Figure 5

3^′-UTR reporter assays confirming the interaction of individual miRNA with the 3^′-UTR of candidate target genes. (A) Schema of the pmirGLO dual luciferase vector carrying the 3^′-UTR regions of 6 selected genes. The entire 3^′-UTR of 6 genes: CDH11, CFL2, SEC23A, ZEB-1, PTPRM, and LDHB were cloned into the pmirGLO dual luciferase vector. (B) The alignments of miRNA and their predicted target genes. (C) Percent relative luciferase activity 48 hours post-transfection with the indicated reporter vector.