Figure 4

Integrative analysis of mRNA and miRNA expression in breast cancer cells. (A) Heatmap representing the differentially expressed genes in miR-200c, miR-205, and miR-375 mimic transfected MDA-MB-231 cells. (B) Venn diagrams showing the intersection between: green circle, mRNA transcripts displaying at least a 1.4-fold decrease in expression in miR-200c, miR-205, and miR-375 mimic transfected MDA-MB-231 cells; blue circle, mRNA transcripts (p < 0.05 and fold change >1.5) that showed an increased expression in aggressive vs. normal and less aggressive cell lines. red circle, likely mRNA targets for miR-200c, miR-205, and miR-375 predicted using TargetScan6.2. The grey area indicates the intersection of all 3 circles. (C-E) qRT-PCR analysis of 11 candidate target genes of miR-200c (C), miR-205 (D), and miR-375 (E) in miR-200c, miR-205, and miR-375 transduced breast cancer cell lines, BT549, HS578T, MDA-MB-231 and SUM159, respectively.