Figure 3

Differential gene expression in 12 breast cancer cell lines. (A) A volcano plot shows that 2412 genes were differentially expressed (p < 0.05 and fold-change >1.5) between normal and less aggressive cell lines vs. aggressive cell lines. (B) The number of predicted miRNA target genes that were up or down-regulated in aggressive breast cancer cell lines. (C) The percentage of predicted miRNA target genes that were up and down-regulated in aggressive breast cancer cell lines. The number above each bar represents the number of predicted target genes of each miRNA by TargetScan 6.2. (D) Confirmation of 9 miRNA target genes using qRT-PCR in 12 breast cancer cell lines. Fold change was calculated using ΔΔCt method. MCF10A was used as the control and therefore the fold change for MCF10A is 1 in all plots.